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KMID : 1098420220300040264
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Korean Journal of Medicinal Crop Science
2022 Volume.30 No. 4 p.264 ~ p.277
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Radical Scavenging Active Compound Screening Analysis in Extract of Coffea arabica L. Leaves
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Lee Kyoung-In
Pyo Byoung-sik
Choi Chul-Hee
Cha Shang-Wha
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Abstract
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Background: Coffee tree leaves contain bio-active compounds that exhibit various functions. Recently, coffee trees have been cultivated in Korea, mainly in the south. We analytically investigated the antioxidant activity of coffee tree leaves grown in Korea.
Methods and Results: We prepared hot water extracts with a specific extraction time (10 min - 120 min) and ethanol extracts using specific concentration levels (5% - 80%). 1,1-Diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity was investigated using liquid chromatography (LC) analysis. We performed a mass spectrometry (MS) using the identified radical scavenging active compound peaks. We confirmed 7 compounds, including 5-caffeoyl quinic acid, 3-caffeoyl quinic acid, cinnamtannin B-1, mangiferin, 3,4-dicaffeoyl quinic acid, 3,5-dicaffeoyl quinic acid, and 4,5-dicaffeoyl quinic acid. The MS with multiple reaction monitoring (MRM) mode analysis provided a method for analyzing radical scavenging active compounds in coffee tree leaf extracts using LC-MS/MS.
Conclusions: The comparative analysis revealed, ethanol extraction had a higher extraction efficiency of the antioxidant active compounds than the hot water extraction. The optimum thechnique used over 40% ethanol for extracting the highest levels of antioxidant activity from coffee leaves.
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KEYWORD
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Coffea arabica, Coffee leaves, Antioxidant, 1,1-Diphenyl-2-picrylhydrazyl Radical, Liquid Chromatography, Mass spectrometry
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